We often take images of our biological and physical experiments. These images can come from a wide range of instruments, and on many different length scales. Encoded in these images is the data that we wish to extract and measure. What is the best way to obtain this information? How do you get data from an image or video? In all these cases, there are some basic principles of image analysis that do not change. Learning about these fundamentals may guide you on your own scientific quests. Once we make measurements, we can then compare them to many different physical models. Our goal in this course will be to cover some of these fundamentals in both theory and practice, and get our hands dirty in the process. You can and are encouraged to use and bring your own data to this class!